DEVELOPMENT OF TEST FOR SYPHILIS

Authors

  • P.V. Khramtsov Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the RAS

DOI:

Keywords:

syphilis, diagnostics, dot blot assay, carbon nanoparticles

Abstract

Non-instrumental dot blot serological syphilis test based on application of functionalized carbon nanoparticles was developed and optimized. Nitrocellulose membrane sensitized with Treponema pallidum p17 protein served as an immunosorbent. Assay demonstrated 100 % sensitivity and specificity when tested with reference panel of serum. Possibility of detection of antibodies against cardiolipin was also shown.

Author Biography

  • P.V. Khramtsov, Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the RAS
    аспирант, Институт экологии и генетики микроорганизмов УрО РАН (ИЭГМ УрО РАН), 614081, г. Пермь, ул. Голева, 13; ассистент кафедры микробиологии и иммунологии биологического факультета, Пермский государственный национальный исследовательский университет (ПГНИУ), 614990, г. Пермь, ул. Букирева, 15; e-mail: khramtsovpavel@yandex.ru

References

  1. Раев М.Б. Нанобиотехнологии в неинструментальной иммуноаналитике / под. ред. В.А. Демакова, Екатеринбург: УрО РАН, 2012. - 140 с.
  2. 2. Патент РФ № 2327992.
  3. Costello P., Green F. Reactivity patterns of human anticardiolipin and other antiphospholipid antibodies in syphilitic sera // Infect. Immun. - 1986. - Vol. 51. I. 3. - P. 771-775.
  4. Costello P., Green F. Cholesterol effects on the interaction of cardiolipin with anti-cardiolipin antibody // Biochimica et Biophysica Acta (BBA) - Biomembranes. - 1987. - Vol. 896. I. 1. - P. 52-56.
  5. Fialová L., Malbohan I., Malíčková K. Avidity of anticardiolipin antibodies-A factor that could be important for their detection by ELISA methods // J. Applied Biomedicine. - 2014. - Vol. 12. I. 4. - P. 277-284.
  6. Huang Q. [et al.]. Dot-immunogold filtration assay as a screening test for syphilis. // J. Clin. Microbiol. - 1996. - Vol. 34. I. 8. - P. 2011-2013.

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  1. Raev M.B. Nanobiotehnologii v neinstrumental’noj immunoanalitike / pod. red. V.A. Demakova, Ekaterinburg: UrO RAN, 2012. - 140 s.
  2. 2. Patent RF No 2327992.
  3. Costello P., Green F. Reactivity patterns of human anticardiolipin and other antiphospholipid antibodies in syphilitic sera // Infect. Immun. - 1986. - Vol. 51. I. 3. - P. 771-775.
  4. Costello P., Green F. Cholesterol effects on the interaction of cardiolipin with anti-cardiolipin antibody // Biochimica et Biophysica Acta (BBA) - Biomembranes. - 1987. - Vol. 896. I. 1. - P. 52-56.
  5. Fialova L., Malbohan I., Malickova K. Avidity of anticardiolipin antibodies-A factor that could be important for their detection by ELISA methods // J. Applied Biomedicine. - 2014. - Vol. 12. I. 4. - P. 277-284.
  6. Huang Q. [et al.]. Dot-immunogold filtration assay as a screening test for syphilis. // J. Clin. Microbiol. - 1996. - Vol. 34. I. 8. - P. 2011-2013.

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Published

2015-10-14

Issue

No. 2 (2015)

Section

Research: theory and experiment

How to Cite

Khramtsov, P. . (2015). DEVELOPMENT OF TEST FOR SYPHILIS. Perm Federal Research Centre Journal, 2, 67-78. https://journal-dev.icmm.ru/index.php/pscj/article/view/PSCJ2015n2p12